Measurement of Immunoglobulin Free Light Chains in Serum

Measurement of immunoglobulin free light chains in serum.

but the active/latent isoform proportions were similar (Fig. 1A). Platelet aggregation during clotting (6) may have caused these differences. MMP expression was lower in K 2 E plasma than in LH plasma [14 (3) vs 25 (4) ␮g/L; P Ͻ0.05; Fig. 1A, lane 4 vs lane 5]. The concentrations of MMP-9 forms decreased significantly with increasing amounts of K 2 E during PB collection, whereas MMP-2 was incr...

Measurement of free light chains in urine.

Quantitation of serum free light chains.

under usual artificial light conditions, and storage of unproc-essed whole blood without antico-agulants at room temperature on the bench. Additionally, we tested the effect of multiple freeze-thaw cycles. 25(OH)–vitamin D 3 measurements were performed on a Cobas E601 an-alyzer (Roche), using the vitamin D 3 (25-OH) assay as recommended by the manufacturer. Samples from each storage experiment ...

Serum levels of immunoglobulin free light chains in patients with chronic hepatitis C presenting cryoglobulinemia.

Hepatitis C virus (HCV) infects B-lymphocytes, provokes cellular dysfunction and causes lymphoproliferative diseases such as cryoglobulinemia and non-Hodgkin's B-cell lymphoma. In the present study, we investigated the serum levels of kappa and lambda free light chains (FLC) of immunoglobulins and the kappa/lambda FLC ratio in Brazilian patients with chronic HCV infection and cryoglobulinemia. ...

Free immunoglobulin light chains in connective tissue diseases.

The basic immunoglobulin subunit consists of two heavy and two light chains held together by covalent disulphide bonds. In most cases of myeloma and Waldenstrom's disease there is an excess synthesis of light over heavy chains, resulting in Bence Jones proteinaemia and proteinuria. Furthermore, in some cases ("light chain disease"), there is absence of detectable heavy chain synthesis, and the ...